alpha Fetoprotein antibody (C-Term)

Catalog No. ABIN6259817

This Rabbit Polyclonal antibody specifically detects alpha Fetoprotein in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for alpha Fetoprotein antibody (C-Term) (ABIN6259817)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-alpha Fetoprotein Antibody

Binding Specificity: C-Term

Specificity: AFP Antibody detects endogenous levels of total AFP.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AFP, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-alpha Fetoprotein antibody (ABIN6259817)

Wu, Tang, Li, Li, Wang, Bao, Bu: "Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 9473-9479, (2018) (PubMed).

Bao, Wu, Wang, Li, Li, Chen, Wu, Xie, Bu: "Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold." in: International journal of molecular medicine, Vol. 38, Issue 2, pp. 457-65, (2017) (PubMed).

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background:

Description: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2 % ) of the human AFP shows estrogen-binding properties.

Gene: AFP

Molecular Weight: 62-65 kDa

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process